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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
21/10/2019 |
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Data da última atualização: |
21/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
QUINTERO, F. O. C.; PINTO, L. G.; BARSALOBRES-CAVALLARI, C. F.; ARCURI, M. de L. C.; PINO, L. E.; PERES, L. E. P.; MALUF, M. P.; MAIA, I. G. |
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Afiliação: |
Fabíola O. Campo Quintero, Department of Genetics/Institute of Biosciences/Sao Paulo State University - UNESP; Layra G. Pinto, Department of Genetics/Institute of Biosciences/São Paulo State University - UNESP; Carla F. Barsalobres-Cavallari, Department of Genetics/Institute of Biosciences/São Paulo State University - UNESP; Mariana de Lara Campos Arcuri, Department of Genetics/Institute of Biosciences/São Paulo State University - UNESP; Lilian Ellen Pino, Department of Biological Sciences - LCB/Escola Superior de Agricultura 'Luiz de Queiroz' - ESALQ/University of São Paulo - USP; Lázaro Eustáquio Pereira Peres, Department of Biological Sciences - LCB/Escola Superior de Agricultura 'Luiz de Queiroz' - ESALQ/University of São Paulo - USP; MIRIAN PEREZ MALUF, CNPCa; Ivan G. Maia, Department of Genetics/Institute of Biosciences/Sao Paulo State University - UNESP. |
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Título: |
Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plant Cell Reports, v. 37, n.9, p.1257–1268, September 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5?-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool. |
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Palavras-Chave: |
Coffee; Promoter; Seed maturation protein; Seed-specific expression; Tomato. |
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Thesagro: |
Café. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/203217/1/Identificaction-of-a-seed-maturation-protein.pdf
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Marc: |
LEADER 02110naa a2200277 a 4500
001 2113247
005 2019-10-21
008 2018 bl uuuu u00u1 u #d
100 1 $aQUINTERO, F. O. C.
245 $aIdentification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.$h[electronic resource]
260 $c2018
520 $aIn this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5?-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool.
650 $aCafé
653 $aCoffee
653 $aPromoter
653 $aSeed maturation protein
653 $aSeed-specific expression
653 $aTomato
700 1 $aPINTO, L. G.
700 1 $aBARSALOBRES-CAVALLARI, C. F.
700 1 $aARCURI, M. de L. C.
700 1 $aPINO, L. E.
700 1 $aPERES, L. E. P.
700 1 $aMALUF, M. P.
700 1 $aMAIA, I. G.
773 $tPlant Cell Reports$gv. 37, n.9, p.1257–1268, September 2018.
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Registro original: |
Embrapa Café (CNPCa) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
23/10/2019 |
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Data da última atualização: |
13/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
POLITI, F. A. S.; FANTATTO, R. R.; SILVA, A. da; MORO, I. J.; SAMPIERI, B. R.; CAMARGO-MATHIAS, M. I.; FIGUEIREDO, A.; CHAGAS, A. C. de S.; FURLAN, M. |
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Afiliação: |
Flávio Augusto Sanches Politi, UNESP; Rafaela Regina Fantatto, UNESP; Alexander Alves da Silva, UNESP; Isabela Jacob Moro, UNESP; Bruno Rodrigues Sampieri, UNICAMP; Maria Izabel Camargo-Mathias, UNESP; Amanda Figueiredo, UNESP; ANA CAROLINA DE SOUZA CHAGAS, CPPSE; Maysa Furlan, UNESP. |
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Título: |
Evaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Experimental and Applied Acarology, v. 77, p. 601-618, 2019. |
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DOI: |
https://doi.org/10.1007/s10493-019-00368-2 |
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Idioma: |
Inglês |
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Conteúdo: |
The cattle tick Rhipicephalus microplus is a great sanitary problem and causes huge losses to livestock, being a vector of important diseases. The aim of this work was to verify the action of plant-derived material obtained from Tagetes patula on the life cycle of R. microplus, as well as to analyze the action of these extracts on ovary cells of engorged females. In the Adult Immersion Test, the crude ethanolic extract (TpEtOH) showed an efcacy of 99.2% (LC50=18.60 mg mL−1). From the repellency test, it was found that both the TpEtOH and essential oil were 100% efcient on the larval stage. In addition, in the Larval Package Test, a mortality of 98.37% was obtained with TpEtOH (LC50=3.798 mg mL−1). From the microscopic analysis of ovary cells, morphological changes in the chorion and cytoplasm of oocytes were observed, with vacuolization around the germinal vesicle and disorganization of the pedicular cells, suggesting an interference in the normal embryogenic formation of the larvae. These results demonstrate that T. patula extracts interfere at all stages of development of the ixodid, from eggs to adults forms, and have a pronounced repellent efect. In addition, the results of the cytotoxicity assays performed on keratinocytes, as well as previous information on oral and dermal acute toxicity (LD50>4000 mg kg−1), attest that T. patula can be a safe alternative for ectoparasitic control, representing an alternative for development of a formulation used to help control populations of ticks in the feld. MenosThe cattle tick Rhipicephalus microplus is a great sanitary problem and causes huge losses to livestock, being a vector of important diseases. The aim of this work was to verify the action of plant-derived material obtained from Tagetes patula on the life cycle of R. microplus, as well as to analyze the action of these extracts on ovary cells of engorged females. In the Adult Immersion Test, the crude ethanolic extract (TpEtOH) showed an efcacy of 99.2% (LC50=18.60 mg mL−1). From the repellency test, it was found that both the TpEtOH and essential oil were 100% efcient on the larval stage. In addition, in the Larval Package Test, a mortality of 98.37% was obtained with TpEtOH (LC50=3.798 mg mL−1). From the microscopic analysis of ovary cells, morphological changes in the chorion and cytoplasm of oocytes were observed, with vacuolization around the germinal vesicle and disorganization of the pedicular cells, suggesting an interference in the normal embryogenic formation of the larvae. These results demonstrate that T. patula extracts interfere at all stages of development of the ixodid, from eggs to adults forms, and have a pronounced repellent efect. In addition, the results of the cytotoxicity assays performed on keratinocytes, as well as previous information on oral and dermal acute toxicity (LD50>4000 mg kg−1), attest that T. patula can be a safe alternative for ectoparasitic control, representing an alternative for development of a formulation used to h... Mostrar Tudo |
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Palavras-Chave: |
Ectoparasitic control; Natural products; Spotted fever. |
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Thesagro: |
Carrapato. |
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Thesaurus NAL: |
Acaricides; Animal parasites and pests; Livestock; Ticks. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02597naa a2200325 a 4500
001 2113388
005 2023-03-13
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10493-019-00368-2$2DOI
100 1 $aPOLITI, F. A. S.
245 $aEvaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari$bIxodidae).$h[electronic resource]
260 $c2019
520 $aThe cattle tick Rhipicephalus microplus is a great sanitary problem and causes huge losses to livestock, being a vector of important diseases. The aim of this work was to verify the action of plant-derived material obtained from Tagetes patula on the life cycle of R. microplus, as well as to analyze the action of these extracts on ovary cells of engorged females. In the Adult Immersion Test, the crude ethanolic extract (TpEtOH) showed an efcacy of 99.2% (LC50=18.60 mg mL−1). From the repellency test, it was found that both the TpEtOH and essential oil were 100% efcient on the larval stage. In addition, in the Larval Package Test, a mortality of 98.37% was obtained with TpEtOH (LC50=3.798 mg mL−1). From the microscopic analysis of ovary cells, morphological changes in the chorion and cytoplasm of oocytes were observed, with vacuolization around the germinal vesicle and disorganization of the pedicular cells, suggesting an interference in the normal embryogenic formation of the larvae. These results demonstrate that T. patula extracts interfere at all stages of development of the ixodid, from eggs to adults forms, and have a pronounced repellent efect. In addition, the results of the cytotoxicity assays performed on keratinocytes, as well as previous information on oral and dermal acute toxicity (LD50>4000 mg kg−1), attest that T. patula can be a safe alternative for ectoparasitic control, representing an alternative for development of a formulation used to help control populations of ticks in the feld.
650 $aAcaricides
650 $aAnimal parasites and pests
650 $aLivestock
650 $aTicks
650 $aCarrapato
653 $aEctoparasitic control
653 $aNatural products
653 $aSpotted fever
700 1 $aFANTATTO, R. R.
700 1 $aSILVA, A. da
700 1 $aMORO, I. J.
700 1 $aSAMPIERI, B. R.
700 1 $aCAMARGO-MATHIAS, M. I.
700 1 $aFIGUEIREDO, A.
700 1 $aCHAGAS, A. C. de S.
700 1 $aFURLAN, M.
773 $tExperimental and Applied Acarology$gv. 77, p. 601-618, 2019.
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Embrapa Pecuária Sudeste (CPPSE) |
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